AP Biology Students Conduct Human Mitochondrial Sequencing Lab at Cold Spring Harbor Laboratory!

On Thursday, our AP Biology students (in Mrs. DeAngelo’s and Ms. Schwartz’s classes) headed over to the Cold Spring Harbor Laboratory to conduct a Human Mitochondrial Sequencing as part of their evolution unit. They extracted DNA from mitochondria in their cheek cells and were guided on how to amplify a small region of their own mitochondrial DNA by Polymerase Chain Reaction (PCR) and use the product as a template for DNA cycle sequencing.

The students obtain their “finished” sequence through the DNALC Internet site. Computer analysis of the data will be performed at school in the coming weeks using the DNALC’s bioinformatics tool “Sequence Server.” This lab examines Single Nucleotide Polymorphisms (SNPs) in the human mitochondrial genome and uses it as the basis for understanding human evolution and worldwide migration patterns.

Thank you to Mrs. DeAngelo for the write-up

and the photos!

 

Leave a Reply

Fill in your details below or click an icon to log in:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out /  Change )

Google+ photo

You are commenting using your Google+ account. Log Out /  Change )

Twitter picture

You are commenting using your Twitter account. Log Out /  Change )

Facebook photo

You are commenting using your Facebook account. Log Out /  Change )

Connecting to %s